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CD19 antibody

CD19 Reactivity: Mouse FACS Host: Rat Monoclonal 6D5 unconjugated
Catalog No. ABIN135146
  • Target See all CD19 Antibodies
    CD19 (CD19 Molecule (CD19))
    Reactivity
    • 281
    • 148
    • 41
    • 29
    • 19
    • 10
    • 7
    • 5
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    • 2
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    Mouse
    Host
    • 171
    • 130
    • 41
    • 4
    Rat
    Clonality
    • 227
    • 115
    • 2
    Monoclonal
    Conjugate
    • 122
    • 22
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    • 8
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
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    • 2
    • 2
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    • 1
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    • 1
    • 1
    • 1
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    • 1
    This CD19 antibody is un-conjugated
    Application
    • 253
    • 113
    • 70
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    • 51
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    Flow Cytometry (FACS)
    Specificity
    Mouse CD19, Mr 95 kDa
    Characteristics
    Rat Anti-Mouse CD19-UNLB
    Purification
    Purified
    Immunogen
    Mouse CD19-expressing K562 human erythroleukemia cells
    Clone
    6D5
    Isotype
    IgG2a
    Top Product
    Discover our top product CD19 Primary Antibody
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP , Sep - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, SPRD, CY5, PE/CY5.5 and PE/CY7 conjugates 0.2 g/106 cells PACBLU conjugate 0.3 g/106 cells AF647 and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Comment

    In vivo and in vitro functional studies

    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
    Preservative
    Without preservative
    Handling Advice
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    CD19 (CD19 Molecule (CD19))
    Alternative Name
    CD19 (CD19 Products)
    Background
    CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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