CD8 alpha antibody (Biotin)
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- Target See all CD8 alpha (CD8A) Antibodies
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD8 alpha antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS)
- Specificity
- Mouse CD8α, Mr 32-34 kDa
- Characteristics
- Rat Anti-Mouse CD8a-BIOT
- Immunogen
- Spleen cells or thymocyte membranes
- Clone
- 53-6-7
- Isotype
- IgG2a
- Top Product
- Discover our top product CD8A Primary Antibody
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anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 8H8A8 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC, Func Host: Rat Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC Host: Rat Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibody (FITC)CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 FITC
anti-CD8a Molecule (CD8A) antibody (PE)CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 PE
anti-CD8a Molecule (CD8A) (AA 51-150) antibodyCD8A Reactivity: Human, Rat, Chicken FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) (AA 22-182) antibodyCD8A Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 6A9A5 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A Reactivity: Human IHC, StM, ISt Host: Mouse Monoclonal C8-468 unconjugated
anti-CD8a Molecule (CD8A) (AA 28-196) antibodyCD8A Reactivity: Mouse, Rat FACS, IHC, WB, ELISA, ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Block - Reported in literature
- Working Dilutions: Flow Cytometry Purified (UNLB) antibody 2 g/106 cells FITC and BIOT conjugates 2 g/106 cells AF488 conjugate 1 g/106 cells PE, APC, SPRD, PE/TXRD, CY5, PE/CY5.5, PE/CY7, 0.2 g/106 cells APC/CY5.5, APC/CY7, PACBLU, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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In vivo and in vitro depletion of CD8a+ cells
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg in 1.0 mL of PBS/Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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- Target
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- Alternative Name
- CD8a (CD8A Products)
- Background
- In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells.
- Pathways
- TCR Signaling
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