CD8 alpha antibody

Catalog No. ABIN135118

Product Details anti-CD8 alpha Antibody

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Mouse CD8α, Mr 32-34 kDa

Characteristics: Rat Anti-Mouse CD8a-UNLB

Purification: Purified

Immunogen: Spleen cells or thymocyte membranes

Clone: 53-6-7

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 2 g/106 cells FITC and BIOT conjugates 2 g/106 cells AF488 conjugate 1 g/106 cells PE, APC, SPRD, PE/TXRD, CY5, PE/CY5.5, PE/CY7, 0.2 g/106 cells APC/CY5.5, APC/CY7, PACBLU, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vivo and in vitro depletion of CD8a+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 alpha antibody (ABIN135118)

Jordan, Buhrman, Sprague, Moore, Gao, Kappler, Slansky: "TCR hypervariable regions expressed by T cells that respond to effective tumor vaccines." in: Cancer immunology, immunotherapy : CII, (2012) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a (CD8A Products)

Background: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells.

Pathways: TCR Signaling