Phosphotyrosine antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN135059

Product Details anti-Phosphotyrosine Antibody (Alkaline Phosphatase (AP))

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Characteristics: The antibody detects: Phosphorylated tyrosine and phenylphosphate

Purification: Purified

Clone: PY20

Isotype: IgG2b

Application Details

Application Notes: Working Dilution:
ELISA: 1:500-1:1,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Product is supplied as 1.0 mL of stock solution in 50 mM Tris / 0 mM MgCl2 / 50 % Glycerol, pH 8.0, containing 0.1 % NaN3 as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Products

Target Type: Amino Acid

Background: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.