CHEK2 antibody (pThr68)

Catalog No. ABIN129576

Product Details anti-CHEK2 Antibody

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Binding Specificity: AA 64-73, pThr68

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 64-73 of Human CHK2.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appropriate cell lysate or extract.  Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pT68 of CHK2.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CHEK2 antibody (ABIN129576)

Lu, Lou, Peng, Xie, Nghiem, Conney: "Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice." in: Cancer research, Vol. 68, Issue 7, pp. 2523-9, (2008) (PubMed).

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2 (CHEK2 Products)

Background: CHK2 (also known as CHEK2, Protein kinase CHK2 isoform a, and checkpoint-like protein) is a serine/ threonine-protein kinase involved in the control of cell cycle checkpoints and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR.  The SQ/TQ cluster domain, therefore, seems to have a regulatory function.  Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain.  CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.
Synonyms: Serine/threonine-protein kinase Chk2 EC=2.7.11.1 Cds1

Gene ID: 11200

NCBI Accession: NP_001005735

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle