AP3D1 antibody (AA 675-700)

Catalog No. ABIN129555

Supplier Product No.: 600-101-290

Product Details anti-AP3D1 Antibody

Target: AP3D1 (Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1))

Binding Specificity: AA 675-700

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AP3D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-101-290

Supplier: Rockland

Purpose: AP3D1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human AP3D1.

Characteristics: Synonyms: goat anti-AP3D1 antibody, AP-3 complex subunit delta-1, Adapter-related protein complex 3 subunit delta-1, AP-3 complex subunit delta, Delta-adaptin

Purification: The product was affinity purified from antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to AP3D1 by western blotting in the appropriate cell lysate or extract. Multiple isoforms exist for this protein.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-AP3D1 antibody (ABIN129555)

Zheng, Saunders, Sorensen, Waxmonsky, Conner: "Notch signaling from the endosome requires a conserved dileucine motif." in: Molecular biology of the cell, Vol. 24, Issue 3, pp. 297-307, (2013) (PubMed).

Concepcion, Flores-Garcuia, Hamilton: "Multipotent genetic suppression of retrotransposon-induced mutations by Nxf1 through fine-tuning of alternative splicing." in: PLoS genetics, Vol. 5, Issue 5, pp. e1000484, (2009) (PubMed).

Target Details for AP3D1

Target: AP3D1 (Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1))

Alternative Name: AP3D1 (AP3D1 Products)

Background: Background: AP3D1 (also known as Delta-adaptin 3, AP-3 complex delta subunit and Delta-adaptin) is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles.

Gene ID: 8943, 117553580

UniProt: O14617