COX IV antibody (Isoform 1)

Catalog No. ABIN127270

Product Details anti-COX IV Antibody

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Binding Specificity: Isoform 1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects 36 kDa annexin VIII. The ~28-30 kDa cleavage fragments can also be detected in Jurkat cell lysate. Blocking peptide is available separately (AP00292CP-N).

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 32 of human Cox IV.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % glycerol, 0.5 % BSA as stabilizer and 0.01 % thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COX IV (COX4I1 Products)

Background: Cytochrome c oxidase (COX) is a 13-subunit complex spanning the inner mitochondrial membrane and responsible for the terminal reduction of dioxygen to water in the electron transport chain. The three core catalytic units COX-1, -2, -3 are trans-membrane proteins encoded by the mitochondrial genome, whereas the remaining 10 subunits are nuclear encoded and expressed in a tissue-specific manner. The expression of nuclear and mitochondrial subunits of the mitochondrial respiratory chain is thought to be highly coordinated. Cox-4 is believed to regulate COX activity according to the extramitochondrial ATP/ADP ratio.Synonyms: COX IV-1, COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, mitochondrial Cytochrome c oxidase subunit 4 isoform 1

Gene ID: 1327

NCBI Accession: NP_001852

UniProt: P13073

Pathways: Proton Transport