HDAC6 antibody (N-Term)

Catalog No. ABIN127242

Product Details anti-HDAC6 Antibody

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody detects ~134 kDa HDAC-6. This antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide mapping to the N-terminus of mouse HDAC-6.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 20 μg/mL. Positive Control: Mouse small intestine tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for longer.

Target Details for HDAC6

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Alternative Name: HDAC6 (HDAC6 Products)

Background: Human HDAC6 (1215 a.a. residues) possesses two separate putative catalytic domains, both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.Synonyms: HD6, Histone deacetylase 6, JM21, KIAA0901

Gene ID: 10013

NCBI Accession: NP_006035

UniProt: Q9UBN7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling