VASP antibody (pSer239)

Catalog No. ABIN126912

Product Details anti-VASP Antibody

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Binding Specificity: pSer239

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: AM00156PU-N recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, mab AM00156PU-N (clone 16C2) is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.

Characteristics: Synonyms: Vasodilator-stimulated phosphoprotein

Purification: Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 239 RKVpSKQE

Clone: 16C2

Isotype: IgG1

Application Details

Application Notes: ELISA (0.05 μg/mL). Immunoblotting (0.5 μg/mL for HRPO/ECL detection). Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Flow Cytometry. Immunoprecipitation (1-10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1-10 μg/mL, may tolerate 0.5 % formaldehyde fixation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 2x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!

Expiry Date: 12 months

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP (VASP Products)

Background: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein

Gene ID: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation