STAT6 antibody (pTyr641)

Catalog No. ABIN126901

Product Details anti-STAT6 Antibody

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Binding Specificity: pTyr641

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically recognizes activated STAT6 phosphorylated at Tyr641 at 100 kDa. It does not cross-react with non-phosphorylated form of STAT6 nor with unrelated phosphorylation sites.

Characteristics: Synonyms: STAT-6, IL-4 Stat, IL-4-STAT, Signal transducer and activator of transcription 6

Purification: Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjuagted to KLH

Clone: 1-60E-013

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for STAT6

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Alternative Name: STAT6 (STAT6 Products)

Background: The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT6 is activated primarily by IL-4 and IL-13. Upon activation, STAT6 is phosphorylated at tyrosine 641 by Janus Kinase (JAK). Phosphorylated STAT6 forms head-to-tail heterodimers and translocates to the nucleus where it paricipates in transcriptional control.Synonyms: IL-4 Stat, Signal transducer and activator of transcription 6

Gene ID: 6778

UniProt: P42226

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response