STAT1 antibody (phosphorylated)

Catalog No. ABIN126895

Product Details anti-STAT1 Antibody

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Binding Specificity: phosphorylated

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically recognizes STAT1 phosphorylated at Ser 727. The antibody does not crossreact with the non-phosphorylated form of STAT1 nor with unrelated serine-phosphorylated proteins.

Characteristics: Synonyms: STAT-1, STAT91, Signal transducer and activator of transcription 1-alpha/beta, Transcriptionfactor ISGF-3 components p91/p84

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjuagted to KLH

Clone: 12C5

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 0.1 mg/mL in in PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in alliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1 (STAT1 Products)

Background: The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT1 is activated by phosphorylation at serine 727. The phosphorylation state of Ser 727 regulates transcription and apoptosis. STAT1 can bind to DNA as heterodimer with STAT3.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events