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Phosphotyrosine antibody (phosphorylated)

Name: Phosphotyrosine antibody (phosphorylated)
SKU: ABIN126878
Price: 684 USD
Availability: InStock

WB, IP, EIA Host: Mouse Monoclonal 16F4 unconjugated

Western Blotting (WB) image for anti-Phosphotyrosine (phosphorylated) antibody (ABIN126878) (Phosphotyrosine antibody (phosphorylated))

1 image

Catalog No. ABIN126878

Datasheet as PDF

Target See all Phosphotyrosine products

Phosphotyrosine
Binding Specificity
22

2

1

1

1

1

1

1

1
phosphorylated

Reactivity

Please inquire
Host
84

27

1
Mouse
Compatible Secondaries
Clonality
94

18
Monoclonal
Conjugate
35

17

10

8

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
Application
79

59

38

38

32

22

21

17

13

9

7

7

7

6

3

2

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Purification

Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components

incl. positive Control

Immunogen

Synthetic phosphopeptide conjugated to KLHEpitope: . . . D-I-pY-A-E . . .

Clone

16F4

Isotype

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, FACS, ICC Host: Mouse Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 FITC

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Atto 594

anti-Phosphotyrosine antibody (Biotin)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Biotin

anti-Phosphotyrosine antibody (PE)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 PE

Application Notes

ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 1-10 μg per 10^6 pervanadate-treated A431 cells. Included Positive Control: Phosphotyrosine MW standard.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore with 1 mL H2O (15 min, RT).

Buffer

1 mL2 x PBS with 0.09 % Sodium Azide/PEG and Sucrose.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Target

Phosphotyrosine

Abstract

Phosphotyrosine Products

Target Type

Amino Acid

Background

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.