Phosphoserine antibody (phosphorylated)

Catalog No. ABIN126865

Product Details anti-Phosphoserine Antibody

Target: Phosphoserine

Binding Specificity: phosphorylated

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Purification: Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: synthetic phosphopeptide conjugated to KLH.

Clone: 16B4

Isotype: IgM

Application Details

Application Notes: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING! ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive control: pSer / pThr Molecular Weight MarkerFormulation: The pSer/pThr molecular weight marker contains rabbit muscle phosphoproteins isolatedby Fe3+/IDA - affinity chromatography. Proteins are lyophilized fromPBS/NaF/PEG/Sucrose/ Bromophenolblue and Na - azide. After reconstitution the solutioncontains 0. 09% Na-azide. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The pSer/pThr molecular weight marker is recommended for immunoblot applications. Use20µl molecular weight marker per lane. Note: Use BSA based blot incubation buffers. Milk,Casein and Blotto might interfere with antibody - antigen interaction. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL H2O (15 min, RT).

Buffer: 1 mL2x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 1 week.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphoserine

Target: Phosphoserine

Abstract: Phosphoserine Products

Target Type: Amino Acid

Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence!