CREB1 antibody (pSer133)

Catalog No. ABIN126754

Product Details anti-CREB1 Antibody

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Binding Specificity: pSer133

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically recognizes CREB phosphorylated at serine 133 and ATF1 phosphorylated at serine 63 in Western blot and ELISA applications.

Characteristics: Synonyms: cAMP Response Element-Binding protein, CREB1, CREB-1, Cyclic AMP-dependenttranscription factor ATF1

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH.

Clone: 1-00E-010

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1 (CREB1 Products)

Synonyms: creb antibody, CREB1 antibody, Creb1 antibody, CREB antibody, 2310001E10Rik antibody, 3526402H21Rik antibody, AV083133 antibody, Creb antibody, Creb-1 antibody, creb1 antibody, zgc:55598 antibody, cAMP responsive element binding protein 1 antibody, cAMP responsive element binding protein antibody, cAMP responsive element binding protein 1 S homeolog antibody, cAMP responsive element binding protein 1a antibody, CREB1 antibody, creb1 antibody, CREB antibody, Creb1 antibody, creb1.S antibody, creb1a antibody

Background: The transcription factor CREB activates transcription in response to a variety of extracellular signals. Various kinases, including Protein kinase A (PKA) , and CaM kinase II, regulate CREB activity by phosphorylation of serine 133. Molecular Weight: CREB:43 kDa, ATF1:38 kDaSynonyms: CREB-1, CREB1, Cyclic AMP-dependent transcription factor ATF1, cAMP Response Element-Binding protein

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation