CDH15 antibody (Extracellular Domain)

Catalog No. ABIN126735

Product Details anti-CDH15 Antibody

Target: CDH15 (M-Cadherin (CDH15))

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDH15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Characteristics: Synonyms: Muscle cadherin, Cadherin-14, CDH15, CDH14, CDH3, Cadherin M, M-Cadherin

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogen: Recombinant extracellualr domain of murine M-cadherin

Clone: 12G4

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunocytochemistry: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CDH15

Target: CDH15 (M-Cadherin (CDH15))

Alternative Name: Cadherin-15 (CDH15 Products)

Background: Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin. M-cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation.Synonyms: CDH14, CDH15, CDH3, Cadherin M, Cadherin-14, M-Cadherin, Muscle cadherin

Molecular Weight: 130 kDa

UniProt: P33146