beta Amyloid antibody (C-Term, N-Term)

Catalog No. ABIN126725

Product Details anti-beta Amyloid Antibody

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Binding Specificity: AA 1-42, C-Term, N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Amyloid antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically interacts with the C-Terminus of beta-Amyloid (1-42) and does not crossreact with beta-Amyloid (1-40).

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: C-terminal peptide (aa 1-42) of Amyloid beta A4 - conjugated to KLH

Clone: 8G7

Isotype: IgG1

Application Details

Application Notes: ELISA: 0.05 μg/mL. Immunoblotting: 1.0 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween20 based blocking and blot incubation buffer. Immunocytochemistry: 0.1-1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS/0.09 % Sodium Azide/PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -20 °C to -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may bestored at 2-8 °C up to 3 months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: Amyloid beta (Abeta Products)

Synonyms: AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN-II antibody, PN2 antibody, aaa antibody, abeta antibody, abpp antibody, ad1 antibody, appi antibody, ctfgamma antibody, cvap antibody, pn2 antibody, Abeta antibody, Abpp antibody, Adap antibody, Ag antibody, Cvap antibody, E030013M08Rik antibody, betaApp antibody, amyloid beta precursor protein antibody, amyloid beta (A4) precursor protein antibody, APP antibody, app antibody, App antibody

Background: The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).Synonyms: Amyloid beta peptide

Pathways: Inflammasome