CD16 antibody

Catalog No. ABIN126000

Product Details anti-CD16 Antibody

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Western Blotting (WB)

Specificity: The antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.

Purification: Affinity Chromatography on Protein-A.

Sterility: 0.2 μm filtered

Immunogen: Human granulocytes

Clone: MEM-154

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 5-10 mg/mL. Positive control: PBL (peripheral blood lymphocytes). Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Immunoprecipitation. Western Blotting (Non-reducing conditions). Functional Application: The antibody MEM-154 blocks binding of human IgG to FcgRIII.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free PBS, pH ~7.4 preserved by 0.2 μm filter sterilization.

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Background: CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process