HSP70 antibody (C-Term)

Catalog No. ABIN125828

Product Details anti-HSP70 Antibody

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Binding Specificity: C-Term

Reactivity: Rainbow Trout

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 70 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Application Details

Application Notes: 1:15 000 with standard ECL (WB), 5 μg of antibodies in reaction mixture (IP), 1: 100 (IHC)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 (HSP70 Products)

Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Molecular Weight: 70 kDa

UniProt: B5X4Z3