CD8 antibody (FITC)

Catalog No. ABIN120695

Product Details anti-CD8 Antibody (FITC)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The antibody recognizes a conformationally-dependent epitope of CD8 antigen (does not react with formaldehyde-fixed cells, negative in Western Blotting application).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Immunogen: Crude thymus membrane fraction.

Clone: MEM-31

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Human blood cells using 20 μL reagent / 100 μL whole blood or 10^6 cellsin a suspension. Does not work in Immunohistochemistry on formalin fixed cells or Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) containing 15 mM Sodium Azide and 0.2 % (w/v) high-grade protease free BSA as a stabilizing agent.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C. DO NOT FREEZE. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

UniProt: P01732