CD43 antibody

Catalog No. ABIN120595

Product Details anti-CD43 Antibody

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: The antibody recognizes human neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Human T lymphocytes

Clone: MEM-59

Isotype: IgG1

Application Details

Application Notes: Western blotting (non reducing conditions). Immunoprecipitation. Flow cytometry. Immunohistochemistry on paraffin sections. Functional applications (activates T lymphocytes. CD43 antibody ABIN120595 induces apoptosis in hemopoietic progenitor cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-CD43 antibody (ABIN120595)

Kosina, Plisek, Dostal, Moravkova, Cermak, Preis, Lukes, Kracmarova, Krausova: "[Invasive streptococcal infections]" in: Klinická mikrobiologie a infekc?ní lékar?ství, Vol. 13, Issue 6, pp. 220-4, (2008) (PubMed).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 / Leukosialin (SPN Products)

Background: CD43 is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity