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CD11b antibody

ITGAM Reactivity: Rat FACS, IP, IHC (fro), Func, IHC (p) Host: Mouse Monoclonal OX-42 unconjugated

Catalog No. ABIN120413

Datasheet as PDF

Target See all CD11b (ITGAM) Antibodies

CD11b (ITGAM) (Integrin alpha M (ITGAM))
Reactivity
265

176

66

31

20

19

17

14

11

10

7

4

4

2

2

1
Rat
Host
148

111

106

2

1

1
Mouse
Compatible Secondaries
Clonality
270

95

2
Monoclonal
Conjugate
122

41

29

27

19

9

7

7

5

5

5

5

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This CD11b antibody is un-conjugated
Application
280

86

81

79

59

45

37

25

23

19

14

14

8

6

5

5

4

4

3

2

2

2

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

Affinity chromatography on Protein G

Immunogen

Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.

Clone

OX-42

Isotype

IgG2a

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ITGAM Reactivity: Human FACS, WB, IHC (p) Host: Rabbit Polyclonal RB17991 unconjugated

anti-Integrin alpha M (ITGAM) (AA 145-340) antibody
ITGAM Reactivity: Human IHC, WB, IF Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) (AA 77-180) antibody
ITGAM Reactivity: Human, Mouse, Rat FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) (AA 31-160) antibody
ITGAM Reactivity: Rat IHC, WB, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) antibody (FITC)
ITGAM Reactivity: Human FACS Host: Mouse Monoclonal MEM-174 FITC

anti-Integrin alpha M (ITGAM) antibody (Biotin)
ITGAM Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-174 Biotin

anti-Integrin alpha M (ITGAM) antibody (PE)
ITGAM Reactivity: Human FACS Host: Mouse Monoclonal MEM-174 PE

anti-Integrin alpha M (ITGAM) (AA 623-728) antibody
ITGAM Reactivity: Human ELISA, ICC Host: Mouse Monoclonal 3A10H5 unconjugated

anti-Integrin alpha M (ITGAM) (AA 941-1074) antibody
ITGAM Reactivity: Human IHC, WB, StM Host: Mouse Monoclonal ITGAM-3340 unconjugated

Application Notes

Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunohistochemistry on paraffin sections. Immunoprecipitation. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.2, 0.09 % Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name

CD11b (ITGAM Products)

Background

The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID

10116

UniProt

Q63001

Pathways

Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation