CD11b antibody (FITC)
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- Target See all CD11b (ITGAM) Antibodies
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD11b antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Purification
- Affinity chromatography on protein G
- Immunogen
- Rat resident peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO/U myeloma cell line.
- Clone
- OX-42
- Isotype
- IgG2a
- Top Product
- Discover our top product ITGAM Primary Antibody
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- Application Notes
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Flow Cytometry: Use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL. Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of ABIN182004 for use in functional studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
- Alternative Name
- CD11b (ITGAM Products)
- Background
- The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
- Gene ID
- 10116
- UniProt
- Q63001
- Pathways
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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