ITGAL antibody (Biotin)

Catalog No. ABIN120404

Product Details anti-ITGAL Antibody (Biotin)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This Antibody is specific for the a subunit of LFA-1. It inhibits homeotypic aggregation of PHA blasts and blocks the binding of rat lymphocytes to purified rat ICAM-1.

Immunogen: Rat Splenic PHA blasts Donor: BALB/c spleen Fusion Partner: Mouse myeloma cell line PAI

Clone: WT-1

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Flow cytometry. Immunohistochemistry (cryostat sections). Functional studies: in vivo/in vitro
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a / ITGAL (ITGAL Products)

Background: LFA-1 (lymphocyte function associated molecule-1) is one of the leukocyte integrins. It is a heterodimer consisting of a and b subunits of 160-170 kDa and 95-100 kDa respectively. LFA-1 promotes non-antigen dependent adhesion of T-cells to a variety of lymphoid cells that bear its complementary receptor I-CAM-1 (1). It has a broad distribution and is found on most common lymphocytes.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

Gene ID: 308995

NCBI Accession: NP_001029170

UniProt: Q3T1L6

Pathways: Activated T Cell Proliferation, Integrin Complex