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CD4 antibody (Domain 1) (FITC)

CD4 Reactivity: Rat FACS Host: Mouse Monoclonal W3-25 FITC

Catalog No. ABIN120375

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Binding Specificity
27

16

16

16

16

10

7

7

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Domain 1
Reactivity
324

200

114

48

24

20

19

18

17

10

10

10

10

10

10

9

9

9

8

5

4

2

1

1

1

1

1
Rat
Host
331

146

110

3

3

1
Mouse
Compatible Secondaries
Clonality
449

137

5
Monoclonal
Conjugate
201

69

62

44

36

12

11

10

10

9

8

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD4 antibody is conjugated to FITC
Application
473

190

125

102

92

71

69

42

39

34

31

28

10

8

8

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Purification

Affinity Chromatography on Protein G.

Immunogen

Rat thymocyte membrane preperation.

Clone

W3-25

Isotype

IgG1

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CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

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CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

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CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Flow cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded Sections. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine- paraformaldehyde) (see Ref. 3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1 mg/mL

Buffer

PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID

10116

UniProt

P05540

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events