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CD4 antibody (Domain 1) (APC)

CD4 Reactivity: Rat FACS Host: Mouse Monoclonal W3-25 APC

Catalog No. ABIN120373

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Binding Specificity
27

16

16

16

16

10

7

7

6

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Domain 1
Reactivity
336

209

120

48

24

20

19

18

17

10

10

10

10

10

10

9

9

9

8

5

4

2

1

1

1

1

1
Rat
Host
352

147

113

3

3

1
Mouse
Compatible Secondaries
Clonality
473

138

5
Monoclonal
Conjugate
205

69

62

44

34

12

11

10

9

9

9

9

8

8

7

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This CD4 antibody is conjugated to APC
Application
497

191

127

102

93

69

69

42

39

34

31

29

10

8

8

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Immunogen

Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Clone

W3-25

Isotype

IgG1

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CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore with 1 mL distilled water

Buffer

PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

RT/4 °C/-20 °C

Storage Comment

Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

CD4 is a 55kD molecule expressed by helper T cells and weakly by monocytes.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID

10116

UniProt

P05540

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events