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CD4 antibody (Domain 1)

CD4 Reactivity: Rat FACS, IHC (fro), IHC (p), Func Host: Mouse Monoclonal W3-25 unconjugated

Catalog No. ABIN120372

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Binding Specificity
27

16

16

16

16

10

7

7

6

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Domain 1
Reactivity
336

209

120

48

24

20

19

18

17

10

10

10

10

10

10

9

9

9

8

5

4

2

1

1

1

1

1
Rat
Host
352

147

113

3

3

1
Mouse
Compatible Secondaries
Clonality
473

138

5
Monoclonal
Conjugate
204

69

62

44

35

12

11

10

9

9

9

9

8

8

7

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This CD4 antibody is un-conjugated
Application
497

191

127

102

93

69

68

42

39

34

30

28

10

8

8

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Purification

Affinity chromatography on Protein G

Immunogen

Rat Thymocyte Membrane Glycoproteins.

Clone

W3-25

Isotype

IgG1

Top Product

Discover our top product CD4 Primary Antibody
anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Functional Assays. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4

Preservative

Azide free

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID

10116

UniProt

P05540

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events