CHO transfected cells expressing the B6 allele of NKG2A. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
KLRC1/2/3
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.Synonyms: CD159a, CD159c, CD159e, NKG2 lectin-like family