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Aurora A antibody

AURKA Reactivity: Human, Mouse WB, IF, IP Host: Mouse Monoclonal 35C1 unconjugated
Catalog No. ABIN119942
  • Target See all Aurora A (AURKA) Antibodies
    Aurora A (AURKA) (Aurora Kinase A (AURKA))
    Reactivity
    • 112
    • 66
    • 37
    • 21
    • 18
    • 12
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Mouse
    Host
    • 107
    • 17
    • 1
    Mouse
    Clonality
    • 100
    • 24
    Monoclonal
    Conjugate
    • 76
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Aurora A antibody is un-conjugated
    Application
    • 90
    • 48
    • 32
    • 24
    • 15
    • 11
    • 10
    • 7
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
    Specificity
    This antibody recognises Aurora-A kinase. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.
    Purification
    Affinity chromatography on Protein G
    Immunogen
    Recombinant Aurora-A. Spleen cells from immunised Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
    Clone
    35C1
    Isotype
    IgG2b
    Top Product
    Discover our top product AURKA Primary Antibody
  • Application Notes
    Immunoprecipitation. Western Blot: 1/500 - 1/1000, this antibody detects a band of approximately 46 kDa in human HeLa and mouse M-ICc12 cell lysates. Immunofluorescence. Recommended Positive Control: Human 293 and mouse LLC1 cell lines.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Aurora A (AURKA) (Aurora Kinase A (AURKA))
    Alternative Name
    Aurora Kinase A (AURKA Products)
    Background
    Aurora-A kinase is a 46 kDa serine/threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6
    Gene ID
    6790
    NCBI Accession
    NP_003591
    UniProt
    O14965
    Pathways
    Cell Division Cycle, Asymmetric Protein Localization
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