Integrin Alpha2b antibody (Biotin)

Catalog No. ABIN119840

Product Details anti-Integrin Alpha2b Antibody (Biotin)

Target: Integrin Alpha2b (CD41)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin Alpha2b antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: This antibody recognizes the integrin alpha IIb subunit CD41. Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo. We recommend the use of SM2035A for use in functional studies.

Purification: Affinity Chromatography on Protein G.

Clone: MWReg30

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1:10, use 10 μL to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline containing, pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin Alpha2b antibody (Biotin) (ABIN119840)

Fujita, Takayama-Tsujimoto, Satoh, Gutiérrez, Aburatani, Fujii, Sarai, Bresnick, Yamamoto, Motohashi: "NF-E2 p45 is important for establishing normal function of platelets." in: Molecular and cellular biology, Vol. 33, Issue 14, pp. 2659-70, (2013) (PubMed).

Takayama, Fujita, Suzuki, Okuyama, Aiba, Motohashi, Yamamoto: "Genetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis." in: Molecular and cellular biology, Vol. 30, Issue 11, pp. 2668-80, (2010) (PubMed).

Perez, Desponts, Parquet, Kerr: "SH2-inositol phosphatase 1 negatively influences early megakaryocyte progenitors." in: PLoS ONE, Vol. 3, Issue 10, pp. e3565, (2008) (PubMed).

Tamagawa-Mineoka, Katoh, Ueda, Takenaka, Kita, Kishimoto: "The role of platelets in leukocyte recruitment in chronic contact hypersensitivity induced by repeated elicitation." in: The American journal of pathology, Vol. 170, Issue 6, pp. 2019-29, (2007) (PubMed).

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: CD41 / ITGA2B (CD41 Products)

Background: Integrin alpha 2b (CD41) is a calcium-dependent, noncovalently associated heterodimer and contains a heavy chain (GPIIb alpha) and a light chain (GPIIb beta) linked by a single disulfide bond. The Integrin alpha 2B chain interacts with the Integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major Integrin on platelets and is important for platelet adhesion and aggregation. CD41 is expressed on platelets and megakaryocytes and forms a heterodimer with CD61. CD41/CD61 is important for platelet adhesion and aggregation.Synonyms: GP2B, GPalpha IIb, ITGAB, Integrin alpha-IIb, Platelet membrane glycoprotein IIb

Gene ID: 16399

NCBI Accession: NP_034705

UniProt: Q9QUM0

Pathways: Integrin Complex