FIV P24 Gag antibody
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- Target
- FIV P24 Gag
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Reactivity
- Feline Immunodeficiency Virus (FIV)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Affinity chromatography on Protein A
- Clone
- PAK3-2C1
- Isotype
- IgG1
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- Application Notes
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Flow Cytometry: Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections. ELISA. Western Blot. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FIV P24 Gag
- Target Type
- Viral Protein
- Background
- The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. The specific binding epitope recognised by clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3 % Triton-X detergent is recommended to reveal this epitope for antibody binding.Synonyms: Feline Immunodeficiency Virus Capsid protein p24
- Gene ID
- 11674
- UniProt
- P16087
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