CD6 antibody

Catalog No. ABIN119554

Product Details anti-CD6 Antibody

Target: CD6

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody reacts with CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).

Clone: MEM-98

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry. Western Blotting under non reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 m g/mL, Positive tissue: spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD6

Target: CD6

Alternative Name: CD6 (CD6 Products)

Background: CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.Synonyms: T-cell differentiation antigen CD6, T12, TP120

Gene ID: 923

NCBI Accession: NP_006716

UniProt: P30203