CD43 antibody (FITC)
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- Target See all CD43 (SPN) Antibodies
- CD43 (SPN) (Sialophorin (SPN))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD43 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognizes the CD43. Clone DFT-1 binds to both glycoforms of human CD43 (Kyoizumi et al. 2004).
- Purification
- Affinity Chromatography on Protein G.
- Clone
- DFT-1
- Isotype
- IgG1
- Top Product
- Discover our top product SPN Primary Antibody
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- Application Notes
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Flow cytometry: use 10 μL of neat antibody to label 100 μL whole blood or 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide, 1 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD43 (SPN) (Sialophorin (SPN))
- Alternative Name
- CD43 / Leukosialin (SPN Products)
- Background
- CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin
- Gene ID
- 6693
- NCBI Accession
- NP_001025459
- UniProt
- P16150
- Pathways
- Regulation of Leukocyte Mediated Immunity
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