CD80 antibody

Catalog No. ABIN119434

Product Details anti-CD80 Antibody

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody recognises the CD80 cell surface antigen, also known as B7-1. Functional studies using Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4 (Morbach et al. 2011).

Purification: Affinity chromatography on Protein A

Clone: MEM-233

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Western blot (non-reducing conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD80

Target: CD80

Alternative Name: CD80 (CD80 Products)

Synonyms: B7 antibody, B7-1 antibody, B7.1 antibody, BB1 antibody, CD28LG antibody, CD28LG1 antibody, LAB7 antibody, B71 antibody, Cd28l antibody, Ly-53 antibody, Ly53 antibody, MIC17 antibody, TSA1 antibody, CD80 molecule antibody, Cd80 molecule antibody, CD80 antigen antibody, CD80 antibody, Cd80 antibody

Background: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. CD80 is expressed by activated B cells, and is important in B cell - T cell interactions, being a ligand of CD28 and CD152 (CTLA-4).Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

Gene ID: 941

NCBI Accession: NP_005182

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints