MUC1 antibody

Catalog No. ABIN119388

Product Details anti-MUC1 Antibody

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognises CD227, the breast cancer associated mucin encoded by the Muc-1 gene. In normal tissues expression is restricted to specialised glandular epithelial. SM1708 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the Mucin.

Purification: Affinity Chromatography on Protein G.

Immunogen: Urinary MUC-1 mucin. Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.

Clone: C595

Isotype: IgG3

Application Details

Application Notes: Flow Cytometry. ELISA: 1/100 - 1/1000. Western Blot. Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin sections, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive control: Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: CD227 / Mucin-1 / MUC1 (MUC1 Products)

Background: MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.Synonyms: Breast carcinoma-associated antigen DF3, CA 15-3, Carcinoma-associated mucin, DF3, EMA, Episialin, H23AG, MUC-1, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

Gene ID: 4582

NCBI Accession: NP_001018016

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling