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His Tag antibody

WB, IP, IHC (fro), EIA Host: Mouse Monoclonal AD1-1-10 unconjugated

Catalog No. ABIN119354

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Reactivity

Please inquire
Host
93

60

11

9

5

4

1

1
Mouse
Compatible Secondaries
Clonality
95

76

10
Monoclonal
Conjugate
79

21

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
143

100

39

27

25

19

15

14

14

13

13

11

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequence

HHHHHH

Purification

Affinity Chromatography on Protein G

Immunogen

PAX6 transcription factor linked to Histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Clone

AD1-1-10

Isotype

IgG1

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Application Notes

Immunhistology on Frozen Sections. ELISA. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4, containing 0.09 % Sodium Azide as a preservative

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

His Tag

Alternative Name

6xHistidine Epitope Tag (His Tag Products)

Target Type

Tag

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.