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His Tag antibody (Biotin)

Name: His Tag antibody (Biotin)
SKU: ABIN119351
Price: 894 USD
Availability: InStock

WB, IP, IHC (fro), EIA Host: Mouse Monoclonal AD1-1-10 Biotin

Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (ABIN119351) and visualised with Streptavidin - HRP (His Tag antibody (Biotin))

1 image

Catalog No. ABIN119351

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Reactivity

Please inquire
Host
92

60

11

9

5

4

1

1
Mouse
Compatible Secondaries
Clonality
94

76

10
Monoclonal
Conjugate
79

21

14

12

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to Biotin
Application
142

100

38

27

25

19

15

14

14

13

13

11

7

5

4

4

4

3

3

3

2

2

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequence

HHHHHH

Purification

Protein G affinity chromatography

Immunogen

PAX6 transcription factor linked to histidine tag

Clone

AD1-1-10

Isotype

IgG1

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anti-His Tag antibody (HRP)
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Application Notes

ELISA: 1/1000. Western Blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is routinely tested in ELISA using histidine tagged ubiquitin and in Western blotting using histidine tagged recombinant protein control.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS with 0.09 % sodium azide as preservative

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

His Tag

Alternative Name

6xHistidine Epitope Tag (His Tag Products)

Target Type

Tag