TLR4 antibody (PE)
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- Target See all TLR4 Antibodies
- TLR4 (Toll-Like Receptor 4 (TLR4))
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Reactivity
- Human, Dog, Pig, Guinea Pig, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TLR4 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Rhesus Monkey, Guinea Pig, Porcine and Canine (Dog).
- Purification
- Affinity Chromatography on Protein G
- Immunogen
- Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- HTA125
- Isotype
- IgG2a
- Top Product
- Discover our top product TLR4 Primary Antibody
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anti-Toll-Like Receptor 4 (TLR4) (AA 751-835) antibody
TLR4 Reactivity: Human, Mouse, Rat, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibodyTLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 49-247) antibodyTLR4 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Alkaline Phosphatase (AP))TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal Alkaline Phosphatase (AP)
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (HRP)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal HRP
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (FITC)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal FITC
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Biotin)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal Biotin
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PE)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal PE
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (APC)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal APC
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PerCP)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal PerCP
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- Application Notes
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Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL distilled water.
- Buffer
- PBS, 0.09 % Sodium Azide, 1 % BSA and 5 % Sucrose
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Prior to and following reconstitution store the antibody at -20 °C.
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- Target
- TLR4 (Toll-Like Receptor 4 (TLR4))
- Alternative Name
- CD284 / TLR4 (TLR4 Products)
- Background
- TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
- NCBI Accession
- NM_138554
- UniProt
- O00206
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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