CD34 antibody (PE)

Catalog No. ABIN119265

Product Details anti-CD34 Antibody (PE)

Target: CD34

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the Murine CD34 cell surface antigen which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognises a Neuraminidase sensitive epitope. As in the Human system, CD34 antibodies in the Mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G.

Clone: MEC14-7

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/5 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.25 mL (SM1603RT) or 1.0 mL (SM1603R) distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative, 5 % Sucrose and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 12490

NCBI Accession: NP_598415

UniProt: Q64314