CD34 antibody

Catalog No. ABIN119263

Product Details anti-CD34 Antibody

Target: CD34

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: This antibody is specific for the CD34 cell surface antigen. It recognises a Neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G.

Clone: MEC14-7

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/10-1/20 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation (2-10 μg/mL). Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. CD34 is a cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 12490

NCBI Accession: NP_598415

UniProt: Q64314