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CD34 antibody (FITC)

CD34 Reactivity: Mouse FACS Host: Rat Monoclonal MEC14-7 FITC

Catalog No. ABIN119261

Datasheet as PDF

Target See all CD34 Antibodies

CD34
Reactivity
274

91

90

21

19

19

19

18

8

7

6

3

2

1

1
Mouse
Host
162

130

18

4
Rat
Compatible Secondaries
Clonality
210

100

3
Monoclonal
Conjugate
126

27

25

20

13

6

6

6

5

5

5

5

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This CD34 antibody is conjugated to FITC
Application
218

179

89

62

52

49

37

37

28

27

23

17

7

6

4

3

3

2

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody recognises the CD34 cell surface antigen. This antibody recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.

Purification

Affinity chromatography on Protein G

Clone

MEC14-7

Isotype

IgG2a

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CD34 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD34 (CD34) antibody (PE)
CD34 Reactivity: Human FACS, ICC Host: Mouse Monoclonal 4H11[APG] PE

anti-CD34 (CD34) antibody
CD34 Reactivity: Human, Rat FACS, IHC, StM Host: Rabbit Monoclonal HPCA1-2598R unconjugated Recombinant Antibody

anti-CD34 (CD34) (C-Term) antibody
CD34 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-CD34 (CD34) (AA 151-385) antibody
CD34 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 Reactivity: Human FACS, WB, IHC (p), ICC Host: Mouse Monoclonal 4H11[APG] unconjugated

anti-CD34 (CD34) (AA 124-375) antibody
CD34 Reactivity: Human WB, IF, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.5 mg/mL

Buffer

PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD34

Alternative Name

CD34 (CD34 Products)

Background

CD34 is expressed by endothelial cells and by haematopoietic stem cells.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID

12490

NCBI Accession

NP_598415

UniProt

Q64314