CDK6 antibody

Catalog No. ABIN119189

Product Details anti-CDK6 Antibody

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises Cyclin Dependent Kinase 6 (Cdk6), a 40 kDa protein which plays an important role in the progression of G1 to S.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: Recombinant CDK6 protein.

Clone: DCS-83-1

Isotype: IgG1

Application Details

Application Notes: Western Blot. Immunoprecipitation. Immunohistochemistry on frozen sections. Recommended positive control tissue: U-2-OS sarcoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDK6

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Alternative Name: CDK6 (CDK6 Products)

Background: Cdk6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.Synonyms: Cell division protein kinase 6, PLSTIRE, Serine/threonine-protein kinase PLSTIRE

Gene ID: 1021

NCBI Accession: NP_001138778

UniProt: Q00534

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases