TSH receptor antibody

Catalog No. ABIN118959

Product Details anti-TSH receptor Antibody

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TSH receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes the Human thyroid stimulating hormone (TSH) receptor binding to an extracellular domain. The epitope has been mapped to aa 379-384 (2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: External domain of Human TSH receptor Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1/AG4.1 myeloma cell line.

Clone: 4C1

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: use 10 μL of 1/100-1/1,000 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections (does not require protein digestion pre- treatment nor antigen retrieval using heat treatment prior to staining of paraffin sections). Histology positive control: Thyroid gland. Immunoprecipitation. Western blotting (1/1,000). According to customer information, this antibody has been used successfully on a peptide microarray (2). Not Suitable for ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TSH receptor

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Alternative Name: Thyrotropin Receptor / TSHR (TSHR Products)

Background: Thyrotropin Receptor, a Glycoprotein Hormone Receptor, plays a central role in controlling thyroid cell metabolism. The TSH receptor can interact with both TSH and autoantibodies against the TSH receptor (thyroid-stimulating immunoglobulins, TSI). Mutations in the receptor are the cause for hyperthyroidism such as gestational hyperthyroidism (hypersensitivity to chorionic gonadotropin) and Grave's disease (autoimmune hyperthyroidism). Multiple isoforms of the thyrotropin receptor are produced by altervative splicing. The thyrotropin receptor has been reported in adipose, adrenal, brain, eye, heart, kidney, skin, thymus, and thyroid. ESTs have been isolated from brain, placenta, and thyroid libraries.Synonyms: LGR3, TSH-R, TSH-receptor, Thyroid-stimulating hormone receptor

Gene ID: 7253

NCBI Accession: NP_000360

UniProt: P16473

Pathways: Thyroid Hormone Synthesis