Transferrin Receptor antibody

Catalog No. ABIN118802

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Affinity Chromatography on Protein G

Immunogen: KGI cell line. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Clone: DF1513

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 10 μL of 1:10-1:25 diluted antibody to label 1x10^6 cells. Immunofluorescence. Immunohistochemistry on frozen tissue sections only (use at 10-20 μg/mL with an avidin-biotin system). Western blot (1-5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / TFRC (TFRC Products)

Background: The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gene ID: 9606

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis