CD72 antibody

Catalog No. ABIN118793

Product Details anti-CD72 Antibody

Target: CD72 (CD72 Molecule (CD72))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD72 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: The antibody reacts with CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family).

Purification: Precipitation methods and ion exchange chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Normal human lymphocytes from a lymph node

Clone: 3F3

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD72

Target: CD72 (CD72 Molecule (CD72))

Alternative Name: CD72 (CD72 Products)

Synonyms: CD72c antibody, Ly-19 antibody, Ly-32 antibody, Ly-m19 antibody, Lyb-2 antibody, CD72b antibody, LYB2 antibody, CD72 antigen antibody, CD72 molecule antibody, Cd72 antibody, CD72 antibody

Background: CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation. CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells, it is also present on follicular dendritic cells.Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32

Gene ID: 971

NCBI Accession: NP_001773

UniProt: P21854

Pathways: BCR Signaling