P-Selectin antibody

Catalog No. ABIN118767

Product Details anti-P-Selectin Antibody

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes CD62P. It reacts with a 140 kDa antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.

Purification: Affinity Chromatography on Protein G

Immunogen: Human platelet membrane glycoproteins.

Clone: AK-6

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/1000-1/5000) and Paraffin Embedded Sections (1/500-1/2000): This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH 6.0. Recommended Positive Control Tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store the product undiluted at 2-8 °C for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62P / P-Selectin (SELP Products)

Background: CD62P (P-Selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.Synonyms: CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP

Gene ID: 6403

NCBI Accession: NP_002996

UniProt: P16109