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CD59 antibody

Name: CD59 antibody
SKU: ABIN118747
Price: 1807.2 USD
Availability: InStock

CD59 Reactivity: Human WB, FACS, IP, IHC (p), IHC (fro), EIA Host: Mouse Monoclonal MEM-43 unconjugated

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD59: AZIDE FREE (ABIN118747). (CD59 antibody)

FACS

1 image

Catalog No. ABIN118747

Datasheet as PDF

Target See all CD59 Antibodies

CD59
Reactivity
143

14

9

8

5

3

2

1

1
Human
Host
88

74

6

6
Mouse
Compatible Secondaries
Clonality
97

74

1
Monoclonal
Conjugate
79

21

15

14

6

4

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This CD59 antibody is un-conjugated
Application
101

69

47

42

33

27

25

16

14

14

13

13

11

11

8

3

3

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity

This antibody reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. MEM-43 has been shown to stimulate NK activity.

Purification

Affinity chromatography on Protein A

Clone

MEM-43

Isotype

IgG2a

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anti-CD59 (CD59) (AA 31-111) antibody
CD59 Reactivity: Human FACS, IHC, ELISA, ICC Host: Mouse Monoclonal 8D2B8 unconjugated

anti-CD59 (CD59) (AA 74-110) antibody
CD59 Reactivity: Human WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB56691 unconjugated

anti-CD59 (CD59) antibody
CD59 Reactivity: Human FACS, IP, IHC (p) Host: Mouse Monoclonal MEM-43 unconjugated

anti-CD59 (CD59) antibody
CD59 Reactivity: Human, Mouse WB, FACS, IP, IHC (p) Host: Mouse Monoclonal MEM-43-5 unconjugated

anti-CD59 (CD59) (AA 31-111) antibody
CD59 Reactivity: Human WB, FACS, IHC, ELISA, IF/ICC Host: Mouse Monoclonal unconjugated

anti-CD59 (CD59) (AA 23-105) antibody
CD59 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD59 (CD59) (AA 1-128) antibody
CD59 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3G6 unconjugated

anti-CD59 (CD59) (AA 26-102) antibody
CD59 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-CD59 (CD59) (AA 52-100) antibody
CD59 Reactivity: Human FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD59 (CD59) antibody
CD59 Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 2E11B6 unconjugated

Application Notes

Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot: The epitope recognised by MEM-43 is lost after reduction, therefore, non- reducing conditions are required for western blotting techniques. Immunohistochemistry on frozen and paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS

Preservative

Azide free

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.
Target

CD59

Alternative Name

CD59 (CD59 Products)

Background

The antigen is expressed on many haemapoietic and non-haemapoietic cells.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin

Gene ID

966

NCBI Accession

NP_000602

UniProt

P13987

Pathways

Complement System