CD56 antibody

Catalog No. ABIN118740

Product Details anti-CD56 Antibody

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises N-CAM expressed on developing and adult neuroectodermal tissues. Neuroectodermal tumours also stain including Glioma, ependymoma, neuroblastoma, medulloblastoma, retinoblastoma and teratoma. Oat cell carcinoma and Wilms tumour are also highly reactive. ERIC-1 will react on Natural Killer cells and recognises 180, 140 and 120 kDa NCAM isoforms.

Purification: Affinity Chromatography on Protein A

Immunogen: Human Retinoblastoma tumour. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the P3/X63.Ag8 mouse myeloma line.

Clone: ERIC-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Western Blot. ELISA: 80 ng/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56 (NCAM1 Products)

Background: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1

Gene ID: 4684

NCBI Accession: NP_000606

UniProt: P13591