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CD47 antibody

CD47 Reactivity: Human, Pig, Cow, Dog, Sheep FACS, IP, IHC (fro) Host: Mouse Monoclonal BRIC-126 unconjugated

Catalog No. ABIN118685

Datasheet as PDF

Target See all CD47 Antibodies

CD47
Reactivity
122

44

25

7

3

3

2

2

2

2

1

1
Human, Pig, Cow, Dog, Sheep
Host
75

65

1

1

1
Mouse
Compatible Secondaries
Clonality
70

68

4
Monoclonal
Conjugate
67

10

10

5

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD47 antibody is un-conjugated
Application
88

77

42

41

32

26

26

18

16

15

14

10

8

6

5

4

3

3

2

2

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

This antibody recognises the CD47 cell surface glycoprotein.

Purification

Affinity Chromatography on Protein G.

Immunogen

Human erythrocytes.

Clone

BRIC-126

Isotype

IgG2b

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anti-CD47 (CD47) (AA 18-135) antibody
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anti-CD47 (CD47) antibody
CD47 Reactivity: Human, Pig, Non-Human Primate FACS, WB, IHC (fro) Host: Mouse Monoclonal MEM-122 unconjugated

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CD47 Reactivity: Human WB, IF, IHC, ELISA Host: Rabbit Monoclonal 4G11 unconjugated Recombinant Antibody

anti-CD47 (CD47) (AA 44-141) antibody
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Application Notes

Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD47

Alternative Name

CD47 (CD47 Products)

Background

CD47 is a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.Synonyms: Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA3

Gene ID

961

NCBI Accession

NP_001768

UniProt

Q08722