FCGR2A antibody
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- Target See all FCGR2A Antibodies
- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FCGR2A antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognises the CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11. SM1092LE is recommended for this purpose.
- Purification
- Affinity Chromatography on Protein A.
- Immunogen
- K562 cell line. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Clone
- AT10
- Isotype
- IgG1
- Top Product
- Discover our top product FCGR2A Primary Antibody
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anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibody
FCGR2A Reactivity: Human WB, IF, IHC, IP, IHC (p) Host: Mouse Monoclonal 9C6 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A Reactivity: Human, Monkey WB, IHC, IP, IHC (p) Host: Mouse Monoclonal 20D1 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 241-317) antibodyFCGR2A Reactivity: Human WB, ELISA, IF, IHC Host: Rabbit Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (C-Term) antibodyFCGR2A Reactivity: Human WB, ELISA, IF, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A Reactivity: Human WB, IF, IHC, IP, IHC (p), ICC Host: Mouse Monoclonal 6H7 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-316) antibodyFCGR2A Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 255-282), (C-Term) antibodyFCGR2A Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB23549 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 256-285), (C-Term) antibodyFCGR2A Reactivity: Human WB Host: Rabbit Polyclonal RB33694 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 1-316) antibodyFCGR2A Reactivity: Human WB Host: Mouse Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 46-150) antibodyFCGR2A Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3E8 unconjugated
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- Application Notes
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Immunoprecipitation: 20 μg/mL. Flow Cytometry (20 μg/mL): Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Recommended Positive Control: Lymph node.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
- Alternative Name
- CD32 / FcRII-A (FCGR2A Products)
- Background
- CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II
- Gene ID
- 2212
- NCBI Accession
- NP_001129691
- UniProt
- P12318
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