CD27 antibody

Catalog No. ABIN118578

Product Details anti-CD27 Antibody

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: The antibody reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Precipitation methods and ion exchange chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human peripheral blood lymphocytes

Clone: LT27

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27 (CD27 Products)

Background: CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.Synonyms: CD27L receptor, T-cell activation antigen CD27, T14, TNFRSF7, Tumor necrosis factor receptor superfamily member 7

Gene ID: 939

NCBI Accession: NP_001233

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints